Experimental Investigation of Laccase Purification Using Aqueous Two-Phase Extraction

Experimental Investigation of Laccase Purification Using Aqueous Two-Phase Extraction

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In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight Mobile Phone Cases of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated.Results show that enzyme activity is Stethoscopes - Binaural Stethoscopes retained during aqueous two-phase extraction.In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration.The partitioning of impurities is not influenced with varying amounts of sodium chloride.